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KMID : 0363120150280020096
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Korean Journal of Pain
2015 Volume.28 No. 2 p.96 ~ p.104
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Anti-allodynic Efficacy of NMDA Antagonist Peptide and Noradrenaline Alone and in Combination in Rodent Neuropathic Pain Model
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Nasirinezhad Farinaz
Hosseini Marjan
Salari Sajad
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Abstract
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Background: The present experiment was conducted to identify the cooperative effect of serine histogranin (SHG) and noradrenaline in alleviating peripheral neuropathic pain.
Methods: Chronic constriction injury of the right sciatic nerve was used to induce chronic neuropathic pain. For drug delivery, a PE10 tube was inserted into the subarachnoid space. Acetone drops and a 44oC water bath were used to evaluate the cold and heat allodynia, respectively. Placing and grasping reflexes were used to assess the locomotor system.
Results: SHG at 0.5 and 1 μg significantly (P ＜ 0.05) decreased the thermal allodynia. The cold allodynia was also significantly reduced by intrathecal injections of 0.5 (P ＜ 0.05) and 1 μg (P ＜ 0.001) of SHG. 1 μg of noradrenaline, but not 0.5 μg, significantly alleviated the cold (P ＜ 0.01) and thermal (P ＜ 0.05) allodynia. The ameliorating effect of noradrenaline or SHG disappeared when the two compounds were administrated in equal concentrations. A significant difference (P ＜ 0.01 in the acetone and P ＜ 0.05 in the heat) was observed in the groups under equal doses of the two compounds, with a lower effectiveness of the combination therapy.
Conclusions: Our findings suggest that the simultaneous administrations of noradrenaline and SHG do not result in synergistic analgesia, and combination therapy may not be a good approach to the treatment of chronic neuropathic pain syndrome.
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KEYWORD
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Antagonists and Inhibitors, Neuropathic pain, Noradrenaline, N-Methyl-D-Aspartate, Receptors
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